Antibiotics by John H. Hash

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By John H. Hash

The severely acclaimed laboratory normal, tools in Enzymology, is without doubt one of the such a lot hugely revered courses within the box of biochemistry. because 1955, every one quantity has been eagerly awaited, usually consulted, and praised by means of researchers and reviewers alike. The sequence includes a lot fabric nonetheless correct this present day - actually a vital booklet for researchers in all fields of lifestyles sciences.

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C. Alkylating Agents . . . . . . . . D. Other Mutagens . . . . . . . . E. Combined Action . . . . . . . . VI. Selection from Mutagen-Treated Populations . . . . . 24 25 26 26 27 29 31 32 35 36 36 I. I n t r o d u c t i o n Strain development, or strain improvement, is a key factor in the development of an antibiotic as a potential therapeutic agent. Before any satisfactory biological testing and chemical characterization of a new antibiotic entity can be accomplished, adequate amounts of the material obviously must be available.

The remainder should be kept available for use in case of an accidental spill, and for submerging glassware on completion of the procedure. 0-ml aliquots distributed into 5 small flasks (or test tubes) labeled 1 to 5 (Table II). Some investigators prefer pregermination of the spore suspension. The 2% N a H C Q solution is added to each flask at the volumes indicated in the table. The HN2 is then added to 4 of the flasks as shown in the table; flask No. 1 is the control. The reaction is usually allowed to proceed for 30 min, preferably with slight agitation (reciprocating shaker).

Mailing, and B. B. Webber, Brookhaven Syrup. Biol. 20, 56 (1967). [2] STRAIN DEVELOPMENT 33 ing agents are carcinostatic, some are also carcinogenic, and safety precautions must be applied in pipetting (use a safety bulb) and disposal. It is beyond the scope of this article to present procedures for the application of many of the alkylating agents as mutagens; while a number have been shown to be effective mutagens in various genetic studies, probably two, the nitrogen mustards and the nitroso compounds, have had the widest application in strain development programs.

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