Electrophoresis by Zdenek Deyl

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By Zdenek Deyl

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Combined methods .................... Disc electrophoresis. . . . . . . . . . . . . . . . . . ................... Immunoelectrophoresis . . . . . . References.. . . . . . . . . . . . . . . . . . . . . . . . . 1. 1 CLASSIFICATIONS OF ELECTROPHORETIC METHODS Criterion of setmation Electrophoretic method Amount separated analytical micropreparative preparative single-step continuous free on carriers capillary gel only migration is involved migration and interaction with carrier migration, interaction with carrier and diffusion low-voltage hig h-voIt age zone moving boundary isotachophoresis focusing methods Type of procedure Method of stabilization Separation principle Potential gradient used Initial and side (marginal) conditions of separation (experimental arrangement) 23 24 26 27 29 31 33 33 33 35 37 24 CLASSIFICATION OF ELECTROMIGRATION METHODS The criteria can be compared only with difficulty and their selection is determined by purpose of a particular classification.

The terminating electrolyte contains an anion with an effective mobility lower than that of any anion in the mixture to be separated. Cations of the terminating electrolyte are not important for the separation. On applying an electric field, separation proceeds until a steady state is established. This steady state is characterized by the fact that individual substances are separated, according to their effective mobilities, into independent sharp, yet close zones. The so-called separated boundaries are localized among the zones.

Particles are oxidized t o a higher soluble oxidation state or material of the electrode is oxidized, giving rise to soluble ions or complexes. The changes that take place on the electrodes and in the solution in which the electrodes are immersed are generally called electrolysis. 49) The proportionality constant A is the so-called electrochemical equivalent, expressed by A = M a (zF)-';M is the molar mass of the separated substance. As a result of electrode reactions, concentration gradients originate near the electrodes, causing diffusion and migration flows.

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